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13 pages, 1674 KiB  
Article
Urban Environmental Predictors of Group Size in Cliff Swallows (Petrochelidon pyrrhonota): A Test Using Community-Science Data
by Cassie Rueda and Kevin J. McGraw
Birds 2025, 6(2), 17; https://doi.org/10.3390/birds6020017 - 3 Apr 2025
Viewed by 47
Abstract
Due to continuing worldwide urban expansion, research into how urban environments affect local flora/fauna has grown significantly. Studies on the impacts of urbanization on birds have explored a wide variety of behaviors (e.g., foraging, breeding, migratory), but there is little research on the [...] Read more.
Due to continuing worldwide urban expansion, research into how urban environments affect local flora/fauna has grown significantly. Studies on the impacts of urbanization on birds have explored a wide variety of behaviors (e.g., foraging, breeding, migratory), but there is little research on the impacts of cities on avian coloniality. Various urban-environmental factors may impact colonial birds. The predominance of impervious surfaces in cities, for example, has been associated with the decline of several bird species due to negative effects on availability and quality of habitat. The urban heat island effect and shifts in resource availability (e.g., food, water) may also affect colonial birds. Here, we used five years of community-science data available in eBird to investigate urban impacts on group size in Cliff Swallows (Petrochelidon pyrrhonota), an abundant colonial bird species that now breeds readily under bridges and other built structures over or near water in Phoenix, Arizona, USA. We hypothesized that, based on the colonial breeding habits of these neotropical migratory birds in this desert environment, swallows in Phoenix would form larger groups in areas with more food and water sources and with more built structures. In fact, we found that proximity to water sources and cropland, but not impervious surface density, was positively and significantly related to group size. These results suggest that, in this desert ecosystem, an abundance of food/water resources provided by humans permits Cliff Swallows to form larger social groups during breeding. Although many studies show harmful impacts of cities on local wildlife, our findings highlight how urban and/or agricultural ‘oases’ may relieve some native species from natural resource limitations and permit them to thrive and increase in group size in human-impacted environments. Full article
(This article belongs to the Special Issue Resilience of Birds in Changing Environments)
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20 pages, 1947 KiB  
Article
Influence of Nesting Habitat and Nest Emplacement on the Breeding Success of the Black Francolin (Francolinus francolinus, Phasianidae): A Case Study from Pakistan
by Asad Ullah, Sumaira Shams, Sultan Ayaz, Eliana Ibáñez-Arancibia, Unays Siraj, Patricio R. De los Rios-Escalante and Farhad Badshah
Birds 2025, 6(2), 16; https://doi.org/10.3390/birds6020016 - 25 Mar 2025
Viewed by 190
Abstract
Limited research exists on the breeding ecology of the black francolin (Francolinus francolinus) in northern Pakistan. This study assessed egg dimensions, clutch size, hatching, fledging, and overall breeding success across different habitats and nests (n = 25) at Totali Game [...] Read more.
Limited research exists on the breeding ecology of the black francolin (Francolinus francolinus) in northern Pakistan. This study assessed egg dimensions, clutch size, hatching, fledging, and overall breeding success across different habitats and nests (n = 25) at Totali Game Reserve, Buner. Generalized linear models (GLMs) were used to analyze the effects of nest site characteristics and nest traits on breeding parameters. Egg dimensions were consistent across sites whereas bush nests had slightly wider eggs. The average clutch size was 5.9 ± 1.7 eggs, with an average of 4.8 ± 1.0 hatchlings per nest. A total of 111 chicks fledged, averaging 4.4 ± 1.0 per nest, yielding an overall breeding success rate of 75.5%. Nests containing six eggs had higher hatching success (76.6%). GLMs results showed a significant positive relationship between clutch size and hatchling, while nest site and traits had no significant effects. However, fledgling success was positively influenced by hatchling numbers, with nests in wetland habitats yielding significantly more fledglings (4.6 ± 0.9) than those from dryland habitats (4.0 ± 1.2). These findings suggest Black Francolins prefer nesting in wetland areas in bushes, likely due to better protection and favorable conditions. Full article
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15 pages, 6173 KiB  
Article
Aerodynamics of Flight Formations in Birds: A Quest for Energy Efficiency
by Fabien Beaumont, Sébastien Murer, Fabien Bogard and Guillaume Polidori
Birds 2025, 6(2), 15; https://doi.org/10.3390/birds6020015 - 24 Mar 2025
Viewed by 205
Abstract
While the formation flight of birds offers numerous benefits, such as reduced predation risk, improved orientation, and enhanced communication, the aerodynamic interactions between birds are not fully understood due to their inherent complexity. This study explores the wake vortex dynamics of two flying [...] Read more.
While the formation flight of birds offers numerous benefits, such as reduced predation risk, improved orientation, and enhanced communication, the aerodynamic interactions between birds are not fully understood due to their inherent complexity. This study explores the wake vortex dynamics of two flying birds and their influence on aerodynamic forces, based on their relative positions in a group. Using a computational finite volume method, the 3D vortex patterns in the wake of Canada Geese (Branta canadensis) flying at 1000 m altitude and 13.9 m/s airspeed were modeled. The results reveal a complex, undulating vortex structure shaped by the wingbeat amplitude and frequency. The analysis shows that trailing birds positioning their wingtips within the upwash region of vortices that are generated by a leading bird significantly reduce drag and enhance lift, achieving up to a 32% improvement in aerodynamic efficiency, calculated as the lift-to-drag ratio. An optimal separation distance of approximately one wavelength (3.47 m) between birds has been identified, leading to a 7% reduction in both mean drag force and aerodynamic power requirements. These findings, extrapolated to larger bird groups, offer valuable insights into the organization and optimal positioning of birds flying in V-formations, advancing our understanding of collective flight dynamics. Full article
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20 pages, 3954 KiB  
Article
A Decadal Change in Shorebird Populations in Response to Temperature, Wind, and Precipitation at Hilton Head Island, South Carolina, USA
by Akshit R. Suthar, Alan R. Biggs and James T. Anderson
Birds 2025, 6(1), 14; https://doi.org/10.3390/birds6010014 - 3 Mar 2025
Viewed by 1246
Abstract
Despite increasing conservation efforts for shorebirds, there are widespread declines in many shorebird species in North America. Climate change is causing significant shorebird range shifts and population declines. This study investigates the relationship between meteorological variability and shorebird population dynamics over ten years [...] Read more.
Despite increasing conservation efforts for shorebirds, there are widespread declines in many shorebird species in North America. Climate change is causing significant shorebird range shifts and population declines. This study investigates the relationship between meteorological variability and shorebird population dynamics over ten years (2014–2023) at Fish Haul Beach, Hilton Head Island, South Carolina, USA. Shorebirds, reliant on specific habitats for breeding and foraging, are increasingly vulnerable to climate-driven changes, including shifts in temperature, precipitation, and wind speed. Using Generalized Additive Models with Poisson distribution, we analyzed species-specific count data for 12 shorebird species in relation to annual meteorological variables. Additionally, the Mann–Kendall test and Sen’s slope were employed to assess decadal trends in population counts. The results reveal significant declines in Black-bellied Plover (Pluvialis squatarola), Marbled Godwit (Limosa fedoa), and Willet (Tringa semipalmata). In contrast, Semipalmated Plover (Charadrius semipalmatus) and Piping Plover (Charadrius melodus) showed increasing trends, indicating potential habitat benefits or conservation success. Temperature emerged as a key driver affecting the abundance of several species, while precipitation and wind speed also played crucial roles in shaping population dynamics. Our findings underscore the sensitivity of shorebird populations to weather fluctuations, emphasizing the need for integrating meteorological variability into management strategies to ensure shorebird conservation. This study provides critical insights into the impacts of meteorological variables on migratory shorebird populations along the Atlantic Flyway. It highlights the importance of maintaining healthy coastal ecosystems in South Carolina. Full article
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16 pages, 1250 KiB  
Article
Bird Collisions with an Unmarked Extra-High Voltage Transmission Line in an Average Riverine Landscape: An Appeal to Take a Closer Look
by Arno Reinhardt, Moritz Mercker, Maike Sabel, Kristina Henningsen and Frank Bernshausen
Birds 2025, 6(1), 13; https://doi.org/10.3390/birds6010013 - 19 Feb 2025
Viewed by 356
Abstract
Anthropogenic structures such as overhead powerlines pose potentially high collision risks to flying animals, particularly birds, leading to millions of fatalities each year. Studies of bird collisions with powerlines to date, however, have estimated different numbers of collision per year and per kilometer [...] Read more.
Anthropogenic structures such as overhead powerlines pose potentially high collision risks to flying animals, particularly birds, leading to millions of fatalities each year. Studies of bird collisions with powerlines to date, however, have estimated different numbers of collision per year and per kilometer in highly variable landscapes. This study aimed to clarify the risk of bird collisions with powerlines in an average landscape, to overcome the bias towards studies in collision hotspots. We conducted experiments to determine searcher efficiency, removal, and decomposition rates of collided birds as well as searching for collision victims and recording flight movements and flight reactions towards the powerlines. Annual bird-strike rates and flight phenology were analyzed using generalized additive models (GAMs). We estimated 50.1 collision victims per powerline kilometer per year and demonstrated that pigeons (especially Wood Pigeon, Columba palumbus) accounted for the largest proportion of collision victims (approximately 65%). Our study thus offers the opportunity to estimate the number of bird collisions (and the range of species) that can be expected in areas that are not particularly rich in bird life or sensitive, especially in view of the planned intensive expansion of energy structures in the context of the green energy transition. Full article
(This article belongs to the Special Issue Bird Mortality Caused by Power Lines)
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16 pages, 4699 KiB  
Article
Spatio-Temporal Characterization of Nest-Niche in Passerine Birds Co-Occurring in Olive Agro-Ecosystems: Partitioning of Nesting Sites, Breeding Chronology, and Productivity
by Wafae Squalli, Ikram Douini, Ismail Mansouri, Hamid Achiban, Hassane Tahiri, Fatima Fadil, Michael Wink and Mohamed Dakki
Birds 2025, 6(1), 12; https://doi.org/10.3390/birds6010012 - 17 Feb 2025
Viewed by 319
Abstract
Many birds regard partitioning feeding and breeding resources as a strategy to escape competition and co-occurring breeding species. During the breeding season, breeding requirements are essential, yet the patterns and drivers of segregation between and within populations are poorly understood. We monitored the [...] Read more.
Many birds regard partitioning feeding and breeding resources as a strategy to escape competition and co-occurring breeding species. During the breeding season, breeding requirements are essential, yet the patterns and drivers of segregation between and within populations are poorly understood. We monitored the habitat use and chronology of the four co-occurring passerines Eurasian Blackbird, European Greenfinch, Common Chaffinch, and European Serin to examine levels of habitat partitioning and success rates during the breeding season. We recorded nesting sites, nest supports, nest placement, breeding chronology, and productivity in four olive orchards in Fez, central Morocco, from March to August 2018–2019. Our results showed substantial spatial segregation in nesting sites, nest supports, and tree vertical placements. Breeding birds showed significant temporal segregation during the nesting, laying, and hatching phases. These findings suggest distinct spatial and temporal differences in habitat selection among co-occurring passerines, which are suggested to increase avoidance of intra and interspecific competition for resources. Breeding success was very high in European Serin and Eurasian Blackbird. Failure factors were recorded: eggs of European Serin were threatened principally by desertion, abortion, and destruction, and eggs of European Greenfinch were impacted by poaching by children. Our findings provide the first data on the spatial and temporal partitioning of breeding resources among passerines species in Northwest African and probably Mediterranean agroecosystems. Full article
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21 pages, 870 KiB  
Article
Monitoring Grassland Bird Communities with Acoustic Indices
by Bethany L. Ostrom, Mary J. Harner and Andrew J. Caven
Birds 2025, 6(1), 11; https://doi.org/10.3390/birds6010011 - 11 Feb 2025
Viewed by 734
Abstract
Several researchers have tried to find relationships between acoustic indices and vocal animal communities to use acoustic indices as a passive monitoring method, as human-derived surveys are expensive, time-consuming, and suffer from observer bias. However, supplanting manual surveys with acoustic indices is a [...] Read more.
Several researchers have tried to find relationships between acoustic indices and vocal animal communities to use acoustic indices as a passive monitoring method, as human-derived surveys are expensive, time-consuming, and suffer from observer bias. However, supplanting manual surveys with acoustic indices is a daunting task, considering effective indices for biological monitoring need to differentiate biologically relevant sounds from the broader soundscape, including anthropophony and geophony. The objective of our study was to test how well acoustic indices can be applied to avian community monitoring within a temperate grassland ecosystem in North America. We collected avian community data and calculated six commonly used acoustic indices from recordings in an intact lowland tallgrass prairie in the Central Platte River Valley of Nebraska throughout the avian breeding seasons of 2019–2021. Singular acoustic indices had only weak correlations with all community metrics. However, multivariate models including multiple acoustic indices showed potential for monitoring grassland bird abundance when anthropophony was considered. Fragmented grassland remnants likely experience significant anthropophony that needs to be accounted for when monitoring avian populations. Additionally, multivariate models incorporating several indices may provide a more accurate prediction of avian biophony than individual acoustic indices. Full article
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25 pages, 2840 KiB  
Review
Birds as Bioindicators: Revealing the Widespread Impact of Microplastics
by Lara Carrasco, Eva Jiménez-Mora, Maria J. Utrilla, Inés Téllez Pizarro, Marina M. Reglero, Laura Rico-San Román and Barbara Martin-Maldonado
Birds 2025, 6(1), 10; https://doi.org/10.3390/birds6010010 - 11 Feb 2025
Viewed by 1314
Abstract
The global crisis of plastic pollution, particularly involving microplastics (MPs) and nanoplastics (NPs), has profound ecological implications. Birds, serving as bioindicators, are especially susceptible to these pollutants. This systematic review synthesizes the current research on the presence, distribution, and impact of MPs and [...] Read more.
The global crisis of plastic pollution, particularly involving microplastics (MPs) and nanoplastics (NPs), has profound ecological implications. Birds, serving as bioindicators, are especially susceptible to these pollutants. This systematic review synthesizes the current research on the presence, distribution, and impact of MPs and NPs on avian species, alongside advancements in detection methodologies. MPs and NPs have been identified in over 200 bird species across 46 families, encompassing several ecosystems, from Antarctica to Labrador, including Australia, China, and South Europe. Seabirds such as penguins, gulls, and shearwaters exhibit a high burden of MPs in tissues and feces due to fishing debris, while terrestrial species face contamination from urban and agricultural sources. Depending on their composition, MPs can cause gastrointestinal damage, oxidative stress, and bioaccumulation of toxic chemicals, particularly polyethylene and polypropylene. However, challenges in detection persist due to methodological inconsistencies, though advances in spectroscopy and flow cytometry offer improved accuracy. Addressing this pollution is vital for bird conservation and ecosystem health, requiring international collaboration and standardized research protocols. Full article
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13 pages, 1025 KiB  
Article
Neither Scale of Response Nor Threshold in Habitat Availability Is Conserved Across Species of Forest-Dwelling Songbirds Responding to Habitat Loss
by Bill A. Thompson
Birds 2025, 6(1), 9; https://doi.org/10.3390/birds6010009 - 10 Feb 2025
Viewed by 450
Abstract
Thresholds can be an effective tool in conservation planning, as they can form a defensible target for habitat conservation or restoration. Generalized thresholds must be used with caution, however, as threshold responses may vary with species and spatial scale. The objectives of this [...] Read more.
Thresholds can be an effective tool in conservation planning, as they can form a defensible target for habitat conservation or restoration. Generalized thresholds must be used with caution, however, as threshold responses may vary with species and spatial scale. The objectives of this study were to identify the scales at which forest-dwelling birds respond to both habitat availability and critical thresholds in forest cover associated with their occurrence, and to assess if life history traits relate to either scale of response or critical threshold. Using point count data from the Ontario Breeding Bird Atlas, I generated concentric buffers ranging from 100 m to 10 km radius around a random subset of point counts and described forest cover and species occurrence within each buffer. I assessed the likelihood of occurrence of each species at each scale of analysis using logistic regression and identified forest cover thresholds below which the occurrence of each species becomes unlikely using fitted regression curves and ROC plots. Species varied in their response to both landscape scale and forest cover, based on relative growth rate, clutch size, and site fidelity. The mean response to forest cover was 30.8%, with landscape scale ranging from 200 m to 9 km. Despite this range, pragmatic approaches to conservation planning are still possible. Full article
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16 pages, 2984 KiB  
Article
Contribution of Seasonal Streams to the Conservation of Native and Migratory Birds in a Coastal Region Undergoing Urbanization
by Catalina B. Muñoz-Pacheco, Javiera C. Gutiérrez and Nélida R. Villaseñor
Birds 2025, 6(1), 8; https://doi.org/10.3390/birds6010008 - 6 Feb 2025
Viewed by 506
Abstract
Urbanization has drastically transformed natural landscapes, threatening biodiversity by reducing species richness and abundance in cities. In this context, seasonal streams serve as refuge areas for various bird species. To determine the role of seasonal streams in conserving native terrestrial avifauna in Algarrobo, [...] Read more.
Urbanization has drastically transformed natural landscapes, threatening biodiversity by reducing species richness and abundance in cities. In this context, seasonal streams serve as refuge areas for various bird species. To determine the role of seasonal streams in conserving native terrestrial avifauna in Algarrobo, an expanding urban environment, we compared bird diversity in ephemeral streams (n = 18) and residential areas (n = 18). Bird counts were conducted during summer and winter. We utilized a diversity index and Generalized Linear Mixed Models (GLMMs) to assess the richness and abundance of native terrestrial birds, as well as the abundance of six migratory birds. Additionally, species accumulation curves verified whether most present species had been adequately sampled. Our results revealed that seasonal streams harbored a greater richness of native bird species than residential areas. Endemic species were also recorded in these streams. The cumulative number of bird species was higher in the seasonal stream environment compared to residential areas. During winter, a higher abundance of migratory birds (Green-backed Firecrown and Gray-hooded Sierra Finch) was observed in seasonal streams. These findings suggest that seasonal streams play a crucial role in conserving native and migratory birds. Thus, protecting and managing these habitats is essential for maintaining avian biodiversity in urbanized areas. Full article
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14 pages, 4403 KiB  
Article
A Comprehensive Microscopy Analysis of the Retina of Larus audouinii (Payraudeau, 1826): Environmental and Ecological Insights
by Alicia Navarro-Sempere, Magdalena García, Raúl Cobo, Sandra Pascual-García and Yolanda Segovia
Birds 2025, 6(1), 7; https://doi.org/10.3390/birds6010007 - 5 Feb 2025
Viewed by 559
Abstract
The retinal structure is generally conserved across vertebrates; however, variations occur due to the relationship between environmental factors and visual perception. Birds have adapted to diverse ecological contexts, evolving specialized visual characteristics. In this study, we examined the retina of Audouin’s Gull ( [...] Read more.
The retinal structure is generally conserved across vertebrates; however, variations occur due to the relationship between environmental factors and visual perception. Birds have adapted to diverse ecological contexts, evolving specialized visual characteristics. In this study, we examined the retina of Audouin’s Gull (Larus audouinii) using light and transmission electron microscopy. This species is particularly intriguing as it is considered an outlier within the Laridae family: despite being a diurnal bird, it exhibits nocturnal/crepuscular foraging behaviour. Our analysis revealed a retina well adapted for diurnal activity, with features such as a retinal thickness comparable to that of other diurnal birds, a well-developed retinal pigment epithelium, and a high cone-to-rod density ratio. However, we also observed characteristics aligned with nocturnal or crepuscular activity, including a slightly increased rod density in central regions and the presence of two distinct types of horizontal cells in the inner nuclear layer. Additionally, the inner retina showed evidence of complex visual processing. These findings suggest that Audouin’s Gull is an excellent model for visual ecology studies due to its specialized adaptations. Full article
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15 pages, 4731 KiB  
Article
Migration Phenology and Spatial Distribution of Soaring Birds in Greece: From Citizen Science Data to Implications for Monitoring and Conservation Strategies
by Anastasios Bounas, Nikos Tsiopelas, Angelos Evangelidis and Christos Barboutis
Birds 2025, 6(1), 6; https://doi.org/10.3390/birds6010006 - 16 Jan 2025
Viewed by 1230
Abstract
Timing and spatial distribution patterns of migratory birds are crucial for their conservation, particularly in Greece, which serves as a vital migratory corridor between Europe, Asia, and Africa. Traditional monitoring methods face challenges due to resource limitations and the country’s complex geography. This [...] Read more.
Timing and spatial distribution patterns of migratory birds are crucial for their conservation, particularly in Greece, which serves as a vital migratory corridor between Europe, Asia, and Africa. Traditional monitoring methods face challenges due to resource limitations and the country’s complex geography. This study aimed to determine the migration phenology and spatial distribution of 18 species of raptors and soaring birds in Greece using citizen science data from eBird, analyzed with generalized additive models (GAMs). We processed 15,940 checklists for spring migration and 9131 for autumn migration from 2010 to 2023. GAMs successfully modeled the migration phenology for most species, revealing variable peak migration dates in spring and more synchronized migration in autumn, with most species migrating in early September. A spatial analysis highlighted the importance of coastal areas and islands (particularly the Aegean islands and Crete) as key migratory routes and stopover sites. Validation with standardized counts from the Antikythira Bird Observatory showed some discrepancies, emphasizing the limitations of relying on a single monitoring site and the value of broad-scale citizen science data. Our findings demonstrate the effectiveness of integrating citizen science data with robust analytical techniques to fill knowledge gaps, providing valuable insights for designing monitoring programs and informing conservation strategies. Full article
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13 pages, 738 KiB  
Article
Literature Review on Mitigation Measures for Bird Electrocutions Occurring Due to Streamers on Transmission Power Lines
by Kaajial Durgapersad, Andreas Beutel and Nishal Mahatho
Birds 2025, 6(1), 5; https://doi.org/10.3390/birds6010005 - 8 Jan 2025
Viewed by 1006
Abstract
Bird streamer faults occur when the streamer, i.e., bird excretion, bridges the gap between two energized components or an energized and an earthed component of a tower structure. This results in a short circuit, which may be accompanied by an outage in the [...] Read more.
Bird streamer faults occur when the streamer, i.e., bird excretion, bridges the gap between two energized components or an energized and an earthed component of a tower structure. This results in a short circuit, which may be accompanied by an outage in the electricity supply. Due to the impact of these faults on electrical infrastructure and bird mortality, a detailed literature review to identify effective mitigation measures for sub-transmission lines (44–132 kV) and transmission lines (132–765 kV) was conducted. The findings show that there are several measures used internationally to reduce streamer-related faults, e.g., bird perch deterrents, shields, changing tower design configurations, changing insulator types and properties, and bird runways. Bird perch deterrents are typically most effectively used by many utilities; however, it is imperative that the perch deterrents are placed and installed correctly. Placement should be above potential problematic areas—to prevent the bridging of the gaps—at various points on different tower structures. Moreover, bird guards need to be monitored and maintained to ensure effectiveness over time. The involvement of a range of stakeholders when making environmental management decisions, such as researching and implementing the best mitigation measures, is also critical to ensure continued success. Full article
(This article belongs to the Special Issue Bird Mortality Caused by Power Lines)
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13 pages, 595 KiB  
Article
The Heterophil-to-Lymphocyte (H/L) Ratio Indicates Varying Physiological Characteristics in Nestlings Compared to Adults in a Long-Lived Seabird
by Vegard Sandøy Bråthen, Dagfinn Breivik Skomsø and Claus Bech
Birds 2025, 6(1), 4; https://doi.org/10.3390/birds6010004 - 3 Jan 2025
Viewed by 862
Abstract
The ratio of the innate heterophils to the acquired lymphocytes (the H/L-ratio) has been advocated as a good indicator of physiological stress. Little is known, however, about their development in wild birds. The present study investigates how leucocyte profiles develop in nestlings of [...] Read more.
The ratio of the innate heterophils to the acquired lymphocytes (the H/L-ratio) has been advocated as a good indicator of physiological stress. Little is known, however, about their development in wild birds. The present study investigates how leucocyte profiles develop in nestlings of a long-lived seabird, the Black-legged Kittiwake (Rissa tridactyla). We counted blood leucocytes in nestlings of about 10 and 25 days of age (n = 23), and in breeding adults around the time of hatching (n = 40) and calculated the H/L-ratio for all three groups. We further investigated if any variation in the leucocyte counts could be explained by body condition or the stress-related hormone corticosterone. 10-day-old nestlings in better body condition showed greater investment in heterophils, which increased their H/L-ratio. The initial focus on innate immunity shifted towards acquired immunity by 25 days, aligning their H/L-ratio with that of adults. In adult Kittiwakes, however, better body condition correlated with lower H/L-ratio, the reverse of the pattern in young nestlings, and this variation was not linked to stress hormone (corticosterone) levels. Overall, our findings suggest that the H/L-ratio reflects the ontogeny of physiological traits and individual condition. As an indicator, values must be interpreted depending on age, in which its sensitivity may also vary. As such, the H/L-ratio may not always be a reliable indicator of physiological stress, in particular related to the release of glucocorticoid hormones. Full article
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12 pages, 274 KiB  
Communication
Molecular and Serological Screening Support the Lack of Coxiella burnetii Circulation in Wild Birds of Portugal
by Filipa Loureiro, João R. Mesquita, Luís Cardoso, Cristina Pintado, Sara Gomes-Gonçalves, Ana C. Matos, Vanessa Soeiro, Andreia Gonçalves, Filipe Silva, Manuela Matos and Ana Cláudia Coelho
Birds 2025, 6(1), 3; https://doi.org/10.3390/birds6010003 - 3 Jan 2025
Viewed by 893
Abstract
Coxiella burnetii is a highly infectious zoonotic pathogenic bacterium that has a major economic impact in the livestock industry throughout the world and causes unpredictable outbreaks in humans worldwide. Although it is known that birds are potential reservoirs of C. burnetii, their [...] Read more.
Coxiella burnetii is a highly infectious zoonotic pathogenic bacterium that has a major economic impact in the livestock industry throughout the world and causes unpredictable outbreaks in humans worldwide. Although it is known that birds are potential reservoirs of C. burnetii, their role in the epidemiological cycle of the pathogen has not been fully verified. Due to its non-specific symptoms and clinical signs, it is certainly an underdiagnosed disease. The objective of this study was to obtain more information on C. burnetii prevalence in wild birds in Portugal. Blood, plasma, and other tissue samples were obtained from wild birds admitted at wildlife rehabilitation centres in Portugal in the scope of passive surveillance. Antibodies specific to C. burnetii were screened using a commercial enzyme-linked immunosorbent assay according to the manufacturer’s instructions. Evidence of C. burnetii infection was sought based on the detection of bacterial DNA. No positive results were found, either in terms of antibodies to C. burnetii or molecular biology. These serological findings do not indicate the endemic circulation of C. burnetii in wild birds, which can be considered relevant information. However, a more complete and serialized approach over time is necessary to be able to make real inferences about the endemicity of the pathogen in the country and its dispersion among wild avian populations. qPCR results were also negative, a finding suggesting that this host population may not play a significant role in the transmission dynamics of C. burnetii. Given the importance of wild bird species as natural reservoirs of this zoonotic bacterium, we consider these data useful for multidisciplinary work in the prevention and control of Q fever, following a One Health approach. Full article
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